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Characterization of BG505 SOS.664-dPG and related Env proteins. ( A ). <t>D7324-capture</t> ELISA with unpurified D7324-tagged BG505 SOS.664, SOS.664-dPG, and BG505 SOSIP.664 trimers expressed in the supernatant of HEK293T cells. ( B ) Top: SEC curve of BG505 SOS.664-dPG produced in suspension 293S cells and purified using 2G12 affinity chromatography. Bottom: BN-PAGE analysis of the SEC fractions depicted above. ( C ) NS-EM analysis on 2G12/SEC-purified BG505 SOS.664-dPG.
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Characterization of BG505 SOS.664-dPG and related Env proteins. ( A ). <t>D7324-capture</t> ELISA with unpurified D7324-tagged BG505 SOS.664, SOS.664-dPG, and BG505 SOSIP.664 trimers expressed in the supernatant of HEK293T cells. ( B ) Top: SEC curve of BG505 SOS.664-dPG produced in suspension 293S cells and purified using 2G12 affinity chromatography. Bottom: BN-PAGE analysis of the SEC fractions depicted above. ( C ) NS-EM analysis on 2G12/SEC-purified BG505 SOS.664-dPG.
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Characterization of BG505 SOS.664-dPG and related Env proteins. ( A ). <t>D7324-capture</t> ELISA with unpurified D7324-tagged BG505 SOS.664, SOS.664-dPG, and BG505 SOSIP.664 trimers expressed in the supernatant of HEK293T cells. ( B ) Top: SEC curve of BG505 SOS.664-dPG produced in suspension 293S cells and purified using 2G12 affinity chromatography. Bottom: BN-PAGE analysis of the SEC fractions depicted above. ( C ) NS-EM analysis on 2G12/SEC-purified BG505 SOS.664-dPG.
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Characterization of BG505 SOS.664-dPG and related Env proteins. ( A ). <t>D7324-capture</t> ELISA with unpurified D7324-tagged BG505 SOS.664, SOS.664-dPG, and BG505 SOSIP.664 trimers expressed in the supernatant of HEK293T cells. ( B ) Top: SEC curve of BG505 SOS.664-dPG produced in suspension 293S cells and purified using 2G12 affinity chromatography. Bottom: BN-PAGE analysis of the SEC fractions depicted above. ( C ) NS-EM analysis on 2G12/SEC-purified BG505 SOS.664-dPG.
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Characterization of BG505 SOS.664-dPG and related Env proteins. ( A ). <t>D7324-capture</t> ELISA with unpurified D7324-tagged BG505 SOS.664, SOS.664-dPG, and BG505 SOSIP.664 trimers expressed in the supernatant of HEK293T cells. ( B ) Top: SEC curve of BG505 SOS.664-dPG produced in suspension 293S cells and purified using 2G12 affinity chromatography. Bottom: BN-PAGE analysis of the SEC fractions depicted above. ( C ) NS-EM analysis on 2G12/SEC-purified BG505 SOS.664-dPG.
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Characterization of BG505 SOS.664-dPG and related Env proteins. ( A ). <t>D7324-capture</t> ELISA with unpurified D7324-tagged BG505 SOS.664, SOS.664-dPG, and BG505 SOSIP.664 trimers expressed in the supernatant of HEK293T cells. ( B ) Top: SEC curve of BG505 SOS.664-dPG produced in suspension 293S cells and purified using 2G12 affinity chromatography. Bottom: BN-PAGE analysis of the SEC fractions depicted above. ( C ) NS-EM analysis on 2G12/SEC-purified BG505 SOS.664-dPG.
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KEY RESOURCES TABLE
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Characterization of BG505 SOS.664-dPG and related Env proteins. ( A ). D7324-capture ELISA with unpurified D7324-tagged BG505 SOS.664, SOS.664-dPG, and BG505 SOSIP.664 trimers expressed in the supernatant of HEK293T cells. ( B ) Top: SEC curve of BG505 SOS.664-dPG produced in suspension 293S cells and purified using 2G12 affinity chromatography. Bottom: BN-PAGE analysis of the SEC fractions depicted above. ( C ) NS-EM analysis on 2G12/SEC-purified BG505 SOS.664-dPG.

Journal: Journal of Virology

Article Title: A modification to heptad repeat 1 of gp41 improves yield and/or quality of soluble pre-fusion HIV-1 envelope glycoprotein trimers

doi: 10.1128/jvi.00913-25

Figure Lengend Snippet: Characterization of BG505 SOS.664-dPG and related Env proteins. ( A ). D7324-capture ELISA with unpurified D7324-tagged BG505 SOS.664, SOS.664-dPG, and BG505 SOSIP.664 trimers expressed in the supernatant of HEK293T cells. ( B ) Top: SEC curve of BG505 SOS.664-dPG produced in suspension 293S cells and purified using 2G12 affinity chromatography. Bottom: BN-PAGE analysis of the SEC fractions depicted above. ( C ) NS-EM analysis on 2G12/SEC-purified BG505 SOS.664-dPG.

Article Snippet: The trimers were then immobilized via their D7324 tags for 2 h at room temperature on half-well 96-well plates (Greiner) precoated with Ab D7324 (Aalto Bioreagents) at 10 μg/mL in 0.1 M NaHCO3, pH 8.6 overnight ( ).

Techniques: Enzyme-linked Immunosorbent Assay, Produced, Suspension, Purification, Affinity Chromatography

KEY RESOURCES TABLE

Journal: Cell reports

Article Title: Centrosome-dependent microtubule modifications set the conditions for axon formation

doi: 10.1016/j.celrep.2022.110686

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Sheep anti-Tubulin (total) , Cytoskeleton , Cat# ATN02; RRID:AB_10708807.

Techniques: Recombinant, Software, Imaging